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Tuesday, July 21, 2020 | History

2 edition of Gas chromatographic analysis of cholesterol in animal tissue. found in the catalog.

Gas chromatographic analysis of cholesterol in animal tissue.

Mara Avots Vaughan

Gas chromatographic analysis of cholesterol in animal tissue.

by Mara Avots Vaughan

  • 140 Want to read
  • 15 Currently reading

Published in Toronto .
Written in English

    Subjects:
  • Cholesterol,
  • Gas chromatography

  • Edition Notes

    ContributionsToronto, Ont. University. Theses (M.Sc.)
    Classifications
    LC ClassificationsLE3 T525 MSC 1968 V38
    The Physical Object
    Pagination[64 leaves]
    Number of Pages64
    ID Numbers
    Open LibraryOL18001077M

    Derivatization, and Gas Chromatography with Tandem Mass Spectrometry. Chapter 9 of. Section B, Methods of the NWQL. Book 5, Laboratory Analysis. Techniques and Methods 5–B9. U.S. Department of the Interior U.S. Geological Survey. THERE has been a considerable amount of recent work on the unsaponifiable fatty material of several types of animal tissue, and one of the results .

    Cholesterol was estimated in fasting serum of the 8 infants fed on humanised milk. Esterified cholesterol was separated by thin-layer chromatography and in it the fatty acid composition was analysed by gas chromatography. Total serum cholesterol was on average , range to , mg% and included mg% esterified cholesterol. In that fraction the ratio of . FOLCH J, LEES M, SLOANE STANLEY GH. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. May; (1)– MIETTINEN TA, AHRENS EH, Jr, GRUNDY SM. QUANTITATIVE ISOLATION AND GAS--LIQUID CHROMATOGRAPHIC ANALYSIS OF TOTAL DIETARY AND FECAL NEUTRAL STEROIDS.

    Animals used for collection of serum were USDA inspected and acceptable: for slaughter. Storage: Store at C: Approval Date and Time: 2/20/ Manufact Date: 1/06/ Expiratn Date: 1/31/ Hoving, EB, Jansen, G, Volmer, M, Van Doormaal, JJ and Muskiet, FAJ () Profiling of plasma cholesterol ester and triglyceride fatty acids as their methyl esters by capillary gas chromatography, preceded by a rapid aminopropyl-silica column chromatographic separation of lipid classes. Journal of Chromatography , pp. –


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Gas chromatographic analysis of cholesterol in animal tissue by Mara Avots Vaughan Download PDF EPUB FB2

Determination of total cholesterol in meat and poultry by gas chromatography: single-laboratory validation. Dinh TT(1), Thompson LD, Galyean ML, Brooks JC, Boylan ML.

Author information: (1)Texas Tech University, Department of Animal and Food Sciences, Lubbock, TXby: 4. For serum cholesterol quantification, at least mL of blood specimen is drawn and around 15 μL of serum is prepared for analysis.

For plant- and animal-derived foods cholesterol determination, a sterol fraction is isolated from lipid extract by thin-layer or column chromatography, following hydrolysis. All these methods commonly share Cited by: 8.

A precise and accurate gas chromatographic method for the determination of cholesterol in processed foods, without prior total lipid extraction, is described.

The accuracy and precision of the method was assessed by using a BCR reference material (anhydrous butter fat). Furthermore, the procedure was compared with an enzymatic by: Capillary liquid chromatography-mass spectrometry with electrospray ionization provides the highest sensitivity in metabolome analysis.

Classical gas chromatography-mass spectrometry is less sensitive but offers extensive structure-dependent fragmentation increasing the specificity in analyses of isobaric isomers of unconjugated bile by: Cholesterol values are generated primarily by gas liquid chromatographic procedures (AOAC ).

Recent meat data has been determined by a. Gas chromatography (GC) is the most common method for analyzing phytosterol content and composition. There are HPLC methods available for separating and quantifying the various forms of phytosterols, such as free sterols, steryl fatty acid esters, steryl glycosides, acylated steryl glycosides, and hydroxycinnamic acid esters of sterols [1,10].

cholesterol content of chicken skin that had been reported up to that date was erroneous, and they reported an analytical value for chicken skin of 71 mg/ g of raw wet tissue. In contrast, data collected in our laboratory (using gas chromatography with flameionizationdetector,GC-FID,forcholesteroldetermination).

Extracted cholesterol was characterised for its purity by gas chromatography (GC) and structure using differential scanning calorimetry (DSC), infrared spectroscopy (IR) and nuclear magnetic resonance spectroscopy (NMR).

The extracted cholesterol can be used for biochemical synthesis. There are several applications of chromatographic science in the field of animal ecology, biology and physiology. One of the most spread uses is identifying the bile acid profiles either by Thin Layer Chromatography, Gas chromatography or High Performance Liquid Chromatography in wild collected feces.

Introduction. Gas chromatography (GC) is a well-established technique used to identify and quantify the incorporation of fatty acids into lipid pools and cell membranes 1,2 during supplementation or physiological conditions such as obesity (and related diseases such as diabetes) or pregnancy It is also suitable for analyzing the types and quantities of fats in.

Gas Chromatography seems to be the most appropriate method for qualitative and quantitative analysis of isolated lipid extracts. Analytical processes for oils like palm oil often use Gas chromatography (GC) with flame ionization detector (GC–FID) method with derivatization.

defined in tests with standard cholesterol solution and 5α-cholestane. The injector and detector analysis temperature was ˚C. The carrier gas used was hydrogen at a constant flow rate of –1, with respective hydrogen and synthetic air flame gas flow rates of 30 –1 and –1.

The analyses were performed by. Cholesterol can be analyzed routinely by gas chromatography (GC)‐flame ionization detector without derivatization; however, other methods, especially high‐performance liquid chromatography (HPLC) coupled with different detectors, can also be used.

[Gas chromatographic determination of cholesterol in tissue]. [Article in German] Mahlberg W. PMID: [PubMed - indexed for MEDLINE] Publication Types: Comparative Study; MeSH Terms.

Adipose Tissue/analysis* Animals; Cholesterol/analysis* Chromatography, Gas/methods; Colorimetry/methods; Liver/analysis* Substances. Cholesterol. Which method involves conversion of a microbe's lipids to volatile compounds for analysis by gas chromatography.

FAME B. proteomic analysis C. MALDI-TOF D. Lancefield group testing. Cholesterol is the most common member of the _____ group, found in animal tissues; it has a tetracyclic carbon ring system with a _____ bond in one of the.

Cholesterol is a constituent of animal tissues that is involved in the develop-ment of atherosclerosis and coronary analysis of tocopherols and sterols are available (Beyer and others ; Smidt Keywords: tocopherols, cholesterol, phytosterols, gas chromatography, food products jfsv67n5pmsBW.p65 7/9/, PM.

that the combined technique of gas chromatography-mass spectrometry is less costly, more versatile and more accessible than formerly. I, therefore, felt that a book on "Gas Chromatography and Lipids" was timely and would complement my book on "High-performance Liquid Chromatography and Lipids", published at the end of MIETTINEN TA, AHRENS EH, Jr, GRUNDY SM.

QUANTITATIVE ISOLATION AND GAS--LIQUID CHROMATOGRAPHIC ANALYSIS OF TOTAL DIETARY AND FECAL NEUTRAL STEROIDS. J Lipid Res. Jul; – LEES RS, HATCH FT.

Sharper separation of lipoprotein species by paper electrophoresis in albumin-containing buffer. J Lab Clin Med. Gas Chromatography: Analysis, Methods and Practices Valerie Warren.

Year: Edition: Unknown Binding. Publisher: Nova Science Publishers. Language: english. Pages: ISBN ISBN File: You can write a book review and share your experiences. Other readers will always be interested in your opinion of the. Analysis by Gas Chromatography In this experiment we will study the method of gas chromatography.

Gas chromatography (GC) is one of the An example of an analysis for cholesterol esters is shown above. If it could be done at all, this separation would take weeks by traditional wet chemistry. Here it took 21 min for the analysis and a few.

For cholesterol determination, samples had first been prepared by the procedure of AOAC () and colorimetrically determined by the assay of Rotenberg & Christensen (). Fatty acid composition The fatty acid compositions of ghee samples were determined by gas chromatography (GC) according to the procedure described by Metcalfe et al.

().Sozen et al., also evaluated the effects of high cholesterol diet on fatty acid, oxysterol and scavenger receptor levels in heart tissues of rabbits, and showed both cholesterol and oxysterols including 4βhydroxycholesterol, 25OHC, 27OHC and 7ketoOHC levels were elevated in heart tissues of high cholesterol fed animals.

Taken together.Major component of all biological membranes; ~25% of total brain lipid is cholesterol. Analysis Note These products are for test and assay use only. They are not meant for administration to humans or animals and cannot be used to diagnose, treat, or cure diseases of any kind.

Other Notes USP issued SDS can be found here.